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Then the perfusion medium was switched to a mixture of 3% ( w / v ) formaldehyde buffered solution (pH 7.4) and 1% ( v / v ) glutaraldehyde solution (150 mL)

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These approaches require medical procedures and carry higher costs and risks

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Furthermore, regulatory approval requires case-by-case evaluation by authorities such as the FDA and European Medicines Agency, emphasising the need for manufacturing consistency and comprehensive safety evaluations [178]

The neurotrophic agents capable of facilitating synaptogenesis and neurogenesis include nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3, and neurotrophin-4 [25,26]