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Reconstitution Fluid and Dilution Reconstitution Fluid: Use sterile or bacteriostatic water (BAC Water)

Gels were transferred to PVDF (polyvinylidene difluoride) membranes followed by probing with primary antibody solutions (1:200 for IGF-1, 1:1000 for IRS-1, phospho-STAT3 and STAT3, and 1:500 for actin) overnight at 4 C

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doi: 10.1136/bmj.324.7353.1560 22

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Place the vial toward the back of the refrigerator where temperature is most stable, not in the door where it swings through temperature changes every time the door opens