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Secondly, the purified GST-STAT3-CCD (218-400), GST-STAT3-DBD (401-564), GST-STAT3-linker (565-663), and GST-STAT3-SH2 (664-768) fragments were bound to glutathione resin as a GST-fusion protein and incubated with anti-GSTP1 for 2 h at 4C

Initial inhibitor screens were conducted in 200 l volumes in 96 well plates with 50 M inhibitor, 100 M NADPH, and 5 nM TGR in 0.1 M potassium phosphate (pH 7.4), 10 mM EDTA with a preincubation step of 15 min

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