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Previous studies suggest that there is a significant relationship between male infertility and a multitude of factors, including hormonal imbalances, genetic abnormalities, varicocele, infections, age, obesity, smoking, alcohol consumption, environmental toxins, and exposure to heat or radiation [16, 17]

Contemporary evidence suggests that bacterial colonization may begin in utero , as indicated by the detection of microorganisms in placental tissue, fetal membranes, amniotic fluid, and umbilical cord blood of healthy mothers and term neonates (203, 204)

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For routine lab handling, keep the reconstituted solution refrigerated at 2-8C and use it within 30 days

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