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The eluate was then incubated with 50 l of anti-FLAG M2 magnetic bead slurry (Millipore, M8823) pre-equilibrated in equilibration buffer according to manufacturers instructions, at 25 C for 2 h with end-over-end rotation
Furthermore, Liang et al
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The combination of the high moisture content of masa and high masa holding temperatures before baking is the likely cause of folic acid chemical degradation when using the traditional method
