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On the 8th day after sowing, two water levels were applied, i.e., normal watering (80 ml pot -1 ) and water deficit (60% of normal watering), designed as W n and W d , respectively, were applied at a 3-day interval based on a preliminary test on soil field capacity, and also based on our previous study

GST-tagged protein purification overview Glutathione-Superflow Resin allows rapid affinity purification of GST-tagged proteins for batch, gravity flow, and FPLC applications

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10.1073/pnas.1416940111 35

And so I just want to make sure that people understand that, hey, look at the science, look at the research, I'm spending everything that I have on doing more trials and doing some more work so that my customers, my family members, my friends, when I talk to them, I can wholeheartedly tell them, this is what we have done, this is what we tried, this is what we worked on, why don't you give it a shot