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The positive control group showed severe damage characterized by disorganized and loosely packed neuronal cells, Purkinje cell hyperplasia, and marked inflammation, reflecting the pathological insults associated with AD

Cells were spun at 4C for 20 min to pellet, and resuspended in lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH = 8)

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