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Elucidating non-monotonic dose-response relationships (NMDR) remains essential for determining accurate low-dose EED toxicity thresholds

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doi: 10.1016/j.immuni.2020.02.017

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To validate the fitting of a 1:1 binding model we carefully ensured that the anisotropy value at the beginning of the direct titrations between Mdm2 and the FAM-labeled peptide did not differ significantly from the anisotropy value observed for the free fluorescently labeled peptide