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in Alcohol and Aldehyde Metabolizing Systems (eds Thurman, R

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J Gastroenterol Hepatol 32(3):609619 Wu CM, Lin CF, Chang YC, Chung TC (2006) Construction and characterization of nisin-controlled expression vectors for use in Lactobacillus reuteri

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doi: 10.1111/j.1438-8677.2012.00677.x

Gradient PCR was used to optimize the annealing temperature of each primer (including endogenous genes -actin and GAPDH primer), followed by agarose gel electrophoresis (1.5%) to visualize the product size of PCR amplified products of target genes