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The supernatants were then collected, aliquoted, and stored at 80C

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Yet, whether and how PVFBs contribute to vessel fibrosis in cSVDs is unknown

Microglia and macrophages in these lesions have a less homeostatic phenotype

Secondary antibodies conjugated to HRP were incubated for 1 to 5 min with ECL Clarity Max (Bio-Rad) for chemiluminescent detection
