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The vial rubber stopper is damaged Repeated punctures through the same spot can core the rubber stopper, creating small rubber particles that fall into the solution

Giblin et al

In some animals, the therapy was administered once at a later time after additional colon obstruction (IC + OB rats)

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While small changes in the scores of these scales might be statistically significant in trials, the impact of such in patients symptoms and quality of life can be meaningless, being important to thoroughly define which score changes are, in fact, impactful [249]