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Transcripts were reconstructed using StringTie (v2.1.0) based on HISAT2 (v2.1.0) alignment, and gene expression levels were calculated using RSEM (v1.2.19) and quantified as FPKM (Fragments Per Kilobase of transcript per Million mapped reads)

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Interestingly, a recent review of all of the known published cases of mitochondrial disease and ASD demonstrated that only about 25% had a known genetic mutation that could account for their mitochondrial disease ( Treatments that are typically used for patients with mitochondrial disease have been shown to improve functioning in some children with ASD (58, 59) and case reports (6063) have reported improvements in core and associated ASD behaviors with l-carnitine treatment

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