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Assessment of DNA fragmentation Sperm DNA fragmentation was evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay using the in situ cell death detection kit (Roche, Mannheim, Germany) [26]

Early studies identified a transcription start site 298 bp upstream of the start codon [34]

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They also protect antigens and adjuvants from enzymatic degradation and ensure efficient uptake by antigen-presenting cells