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However, the role of ferroptosis in aminoglycoside-induced ototoxicity has not been studied in mammals in vivo , and the mechanisms of ferroptosis in regulating ototoxic hair cell death are unclear

Cells were maintained in a humidified incubator at 37 C with 5% CO 2

The mixture was bath sonicated (40 kHz, 70 W) for 2 h

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Situations requiring extra caution Certain populations should exercise additional caution or avoid penta deca peptide arginate entirely