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Rinse thoroughly to avoid residue

At 590 nm, the increase in absorbance was recorded

The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system

As expected, db/db mice which harbors mutated lepRb display a phenotype similar to leptin-deficient ob/ob mice, suggesting that LepRb is the most critical in mediating leptins actions [14]

Tamtaji OR, Heidari-Soureshjani R, Mirhosseini N, Kouchaki E, Bahmani F, Aghadavod E et al (2019) Probiotic and selenium co-supplementation, and the effects on clinical, metabolic and genetic status in Alzheimer's disease: a randomized, double-blind, controlled trial

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