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The gad1b promoter from the transgenic line Tg(gad1b:mCherry) was cloned and inserted into Cas9-T2A-mCherry,U6:gRNA(null) to create the gad1b:Cas9-T2A-mCherry,U6:gRNA(null) vector by Gibson assembly reaction 96

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You may also be asked to go to another location for testing

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720726 The effects of Q loss in bacteria may well be more complex, and strongly growth condition-dependent, but can lead to biased translation and proteome remodeling, particularly under the combined effects of nutritional stress and antibiotic stress