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Depletion of cellular GSH by using buthionine sulfoxamine (BSO), which inhibits glutamatecysteine ligase (the rate-limiting enzyme for GSH synthesis) is sufficient to induce ferroptosis due to impaired cellular antioxidant capacity 18

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However, our knowledge about the mechanisms of CoQ distribution in the cell is still very limited, and it is unknown if the cell responds with a redistribution in the CoQ pool under CoQ deficiency

Flow cytometry Cell cycle analysis of cells cultured in liquid medium was performed as described [28] using a FACS Canto II flow cytometer (BD Bioscienes, San Jose, CA, USA)