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In this study, 18 mice (6/group) were euthanized 12 h after CLP to analyze oxidative stress parameters and 18 mice (6/group) were euthanized 24 h after CLP to analyze pro-inflammatory cytokines based on previous studies (9, 21) (Figure 1)

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334 Supplementation with Icariin reduces ferroptosis markers and attenuates the progression of MASH in mice fed a methionine choline-deficient diet

So, for example, if you made a change and then something improved, look at everything that changed and were they all overlapping, in which case it's kind of hard to figure out what it is, or is there a distinct association, time-wise, between something changing and something improving