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In the subsequent processing, steel beads were added to the mixture, which was then placed in a tissue grinder operating at a frequency of 50 Hz for 120 s to ensure complete homogenization of the sample

The deparaffinized sections were used for immunofluorescence and hematoxylin and eosin staining

B.CancelJ.RoundsM.OchoaV

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Through our in vivo and in vitro studies, we demonstrated that GR deficiency leads to a pronounced reduction in bacterial growth and biofilm formation, and a weakened interaction with host macrophages