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63 N-acetyl cysteine is the antidote to acetaminophen toxicity and a mucolytic agent

Inouye (Sebastopol, CA), Varadarajan Dwarakanath (Houston, TX) Assignee(s): CHEVRON U.S.A

The supernatants of the cell lysates were isolated at 12,500 g for 30 min at 4 C and were incubated with anti-Flag magnetic beads overnight at 4 C, followed by extensive washing with NP-40 Lysis Buffer

First, the sulfonation reaction saturates and that allows more NAPQI to accumulate ([7, 24]) and second, the increased amount of NAPQI exhausts the liver stores of reduced glutathione (GSH)[6] as well as the livers capacity to synthesize new GSH

2020;145 doi: 10.1016/j.fct.2020.111715