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Experiments performed in differentiated SH-SY5Y neuroblastoma cells have shown that exposure to PS-NPs downregulates expression of the CI subunit NDUFB8, leading to diminished basal and maximal oxygen consumption rates alongside increased proton leakagefurther confirming mitochondrial CI dysfunction 32

Methods Chemicals Reduced form of L-glutathione was obtained from KOHJIN Life Sciences (Tokyo, Japan)

enhancing their stability in the physiological environment is a crucial challenge

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doi: 10.3390/cells9071687

Next, to validate the silencing efficiency of the Glut1 gene in PDAC cells, we screened three siG sequences and identified the most effective one for subsequent use (Supplementary Tables 3, 5 and Supplementary Fig