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10.1016/j.ccr.2007.05.008 Cancer Cell 72 Guevara-AguirreJ.BalasubramanianP.Guevara-AguirreM.WeiM.MadiaF.ChengC

RNA extraction and RNA sequencing Before extracting the RNA, we used QIAshredder (#75654, QIAGEN Sciences, Germantown, MD, USA) to homogenize the cell lysates and eliminate any debris

At the cellular level, this peptide acts as a signaling molecule that modulates gene expression across numerous pathways relevant to skin aging and repair

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These approaches enable systematic analysis of immune cell dynamics, cellcell interactions, and pathologic microenvironments within tissues, leading to a more comprehensive understanding of inflammatory phenotypes (Figure 6B) ( in silico pre-screening of natural products, with top candidate compounds subsequently validated in human organoidzebrafish chimera models