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High-throughput assay development, compound treatment, and liquid handling 10 ml pre-cultures were started from single colonies and incubated overnight

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Later the structure was refined, demonstrating that the substance is a tripeptide, -Glu-Cys-Gly [3,4,5]

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Thus, the fermentation processes can lead to either an intracellular or extracellular production of bioactive compounds which can be recovered either as an intact whole cell or cell-free extract using organic solvents
