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a-b , HCC-1806 cells were transduced with CRISPRi lentiviral vector containing sgRNA guides targeting the GGT1 promoter

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a , Scheme of the in vitro sgRNA competition assay performed in HEK-293T SLC25A39 knockout cells expressing a vector control or SLC25A39 cDNA transduced with a pool of 5 control sgRNAs (sgControl, gray) and sgRNAs targeting TXNRD2 (pink) and SLC25A40 (green) (left)

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Note: Originally published in April 2015, this article has been revised and updated for accuracy and thoroughness.

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