is it okay to take glutathione without eating How Effectively: Dosage & Tips DoSe Liposomal Glutathione, 450mg 150ml

Can be used as a once-off booster or mixed in with daily moisturiser

The antioxidant activity of melanoidins isolated from different brews was studied, and some showed that the radical scavenging ability of coffee melanoidins was higher in the dark-roasted coffee, others exhibited higher radical scavenging activity, and still others showed radical scavenging ability of coffee melanoidins was not affected by the degree of roasting (Tagliazucchi, 2010)

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

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Bibliography 1.Takeo.M, Lee.W, Ito.M, Wound Healing and Skin Regeneration