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Bioactive Compounds in Underutilized Fruits and Nuts

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A second cannula (Male Luer to Hose Barb Adapter, 45518-46, Cole Parmer, Vernon Hills, IL) was used to cannulate the inferior vena cava for venous drainage

The culture was lysed in 20ml of binding/wash buffer and loaded onto a column containing 1ml of HisLink Resin