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The detection of anti-HCV antibodies in plasma or serum is based on the use of third-generation EIAs, that detect mixtures of antibodies directed against various HCV epitopes

RNA-seq libraries were prepared with the NEBNext poly(A) Magnetic Isolation Module (NEB #E7490L) followed by the NEBNext Ultra Directional RNA library preparation kit for Illumina (NEB #E7420L) according to manufacturers protocol

The PPT diet was compared to a Mediterranean diet intervention (participants were encouraged to include whole-wheat bread and grains, legumes, fruits, vegetables, olive oil, fish, poultry and low-fat dairy products in their diet) (151)

Be careful not to over-scrub, as this can damage the thin skin barrier and lead to more inflammation