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The supernatant was then analyzed by HPLC with a Rainin Econosphere ODS C18 column and an acetonitrile/water mobile phase containing 0.1% trifluoroacetic acid

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doi: 10.1093/brain/awh622

Mebarki, M., Abadie, C., Larghero, J

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Extract-Based CFE Systems To produce proteins of interest, CFE systems harness an ensemble of catalytic components necessary for transcription, protein biosynthesis, protein folding, and energy regeneration from crude cell extracts