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Assessment of T-cell proliferation T-cells were washed with pre-warmed PBS, resuspended in PBS, loaded with 1 M CFSE and incubated for 15 min at 37C

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The role of GPX4 as the main regulator in the ferroptotic process is based on its unique function to reduce complex hydroperoxides including phospholipid hydroperoxides and cholesterol hydroperoxides to their corresponding counterparts, thereby interrupting the lipid peroxidation chain reaction (Seibt et al., 2019)