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In the case of UreG, this creates a subunit-bridging Ni II site that is coordinated by an invariant Cys -Pro- His sequence from opposite protomers and positioned between the G2 and G3 loops in the primary structure, to form an approximately square planar Cys 2 -His 2 coordination complex (Figure 38A), 822 mutagenesis of which abolishes urease maturation

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Once reconstituted with bacteriostatic water, they should be refrigerated at 28C and used within the recommended timeframe

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By contrast, in the experiments in which the course of ischemia was not reversed, decreased NO levels in the colon tissue appeared as an immediate heavy loss of endothelial cells from the vascular wall, which resulted in a lower eNOS production ability[42]

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