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mtDNA mutations are implicated in various disorders, including mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS), maternally inherited Leigh syndrome (MILS), myoclonic epilepsy with ragged red fibers (MERRF), Lebers hereditary optic neuropathy (LHON), Kearns-Sayre syndrome, Pearson syndrome, and chronic progressive external ophthalmoplegia (CPEO)

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The cells were washed with PBS twice before staining with X-gal solution [1 mg/mL 5-bromo-4-chloro-3inolyl--D-galactoside (Boehringer Manneheim #745-740) in N,N-dimethylformamide, 5 mM potassium ferricyanide crystalline (#P-8131), 5 mM potassium ferricyanide trihydrate (#P-3289), 2 mM magnesium chloride, and 1x PBS, pH 6.0