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16 The aim of this paper is to review the evidence for the use of B12 in clinical practice beyond the replacement in deficiency

Then brain sections were incubated with rabbit monoclonal anti-HDAC9 antibody (Abcam, #ab109446, 1:200) in combination with one of following antibodies: mouse monoclonal anti-NeuN antibody (MilliporeSigma, #MAB377, 1:200), goat polyclonal anti-IBA1antibody (Abcam, #ab5076, 1:200) or mouse monoclonal anti-GFAP antibody (cell signaling technology, #3670, 1:200) diluted in TBST containing 10% donkey serum overnight at 4 C

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