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There is also concern that the addition of glutamate may increase the osmotic pressure of the medium

114 The density of AMPA, NMDA and P2X receptors as well as the density of plasmalemmal glutamate transporter currents demonstrates bell-shaped age dependency, with maximal densities observed in adult animals, whereas ageing is accompanied by a 2-3 fold decrease of density of respective ion currents

10.1016/j.devcel.2019.10.007 Dev

The downstream metabolites of the kynurenine metabolic pathway, quinolinic and kynurenic acids, are neuroactive metabolites acting on the glutamatergic N -metyl-D-aspartate (NMDA) receptor (57)

doi: 10.1038/srep11427

Kluding, P