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Immunocytochemistry Isolated NRCs were plated on Lab-Tek II chamber slides (Thermo Scientific, 154461) at a seeding density of 1 10 5 cells per well

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The effects on total sleep time vary, but each medication in this class will reduce sleep latency by about 20 minutes (Bragg et al., 2019).Melatonin and sedating Histamine-1-Receptor Antagonists (Doxylamine and Diphenhydramine) are two over-the-counter medications that are marketed for the treatment of insomnia, but there is insufficient evidence to support the use of these medications (Silber, 2005)