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For ratiometric measurements of the HyPer reporter the animals were imaged at a Zeiss Meta 710 confocal microscope at an excitation of 405 and 488 nm, while emission was detected at 516 nm

After 4 hours, nonadherent cells were removed, and the culture medium was replaced with Lonza HCM hepatocyte medium (CC-3198) or continued in DMEM with 20% FBS, depending on experimental needs

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Through multivariate statistical analyses (including PCA and PLS-DA), we systematically investigated the remodeling characteristics of amino acid metabolic networks during fistula pathogenesis, aiming to identify the critical nodes driving their distinct etiological mechanisms

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Clinical literature and dermatology reviews consistently describe topical GHK-Cu as highly tolerable