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10.25259/JNRP_356_2023 254 VassilakiM.AakreJ

As the main substrate of lipid peroxidation released from PL in the cell membrane, AA is a precursor of proinflammatory mediators that can be metabolized by cyclooxygenases (COX), ALOX, and CYP450 monooxygenases to synthesize biologically active inflammatory mediators, such as prostaglandins (PGs) and leukotrienes [91]

Handling and contamination prevention Minimize handling of refrigerated peptides

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MitoSOX staining for mitochondrial ROS detection The MitoSOX staining results (Fig