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FIGURE 1 GATases are widely distributed in all kingdoms of life and are used to assimilate nitrogen in biological systems by participating in the biosynthesis of amino acids, nucleic acids, and co-factors (Massire and Badet-Denisot, 1998)

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Although these trigger sites presumably afford greater affinity to the Argonaute (AGO)miRNA complex owing to pairing to the miRNA 3 region 17 , this increased affinity is insufficient to explain the selectivity of the target-directed miRNA degradation (TDMD) machinery for the vastly outnumbered trigger-bound complexes

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