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Galectin-3-positive puncta were imaged at 40 magnification (using an objective water immersion lens) on a Leica DMi8 confocal fluorescence microscope

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The cultures were then labeled 12 h after blocking with 1:500 TNR antibodies (neurons) or 2 g/mL Fab fragments directed against TNR (organotypic slices), together with 1 mg/mL biotinylated FluoTag-X2 secondary anti-mouse secondary nanobodies (custom-made

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Ackley, J