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Everglades City, FL 34139 Amount: $1 million Justification: The existing Master 1 & 2 pump station collects and re-pumps 100% of the wastewater from Chokoloskee Island

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When applied to a peptide sequence designed to interact with the C-terminal domain of the HIV-1 capsid assembly polyprotein (C-CA), this stapling modification resulted in improved binding affinity, cell permeability, and proteolytic stability compared to its unstapled counterpart

Two-fold concentrated stock solutions containing either 2 mM Aldrithriol-2 (Sigma-Aldrich, Darmstadt, Germany, CAS-2127-03-9, AT-2) (oxidation control), 10 mM Dithiothreitol (Sigma-Aldrich, Darmstadt, Germany, CAS-3483-12-3, DTT) (reduction control), 5 mM reduced glutathione (GSH, Sigma-Aldrich, CAS-70-18-8, pH 7.4 in 1 M HEPES), 5 mM oxidized Glutathione (GSSG, Sigma-Aldrich, CAS-27025-41-8, pH 7.4 in 1 M HEPES) or HEPES were prepared freshly