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Three replicates were prepared for each group and incubated at 37 C for 2 h

Micropipettes used in this experiment were made from borosilicate glass (World Precision Instruments) with a pipette puller (PC-10, Narishige) and filled with the RNA containing Intracellular Solution (126 mM K-gluconate, 4 mM KCl, 10 mM HEPES, 2 mM Mg-ATP, 0.3 mM Na 2 -guanosine 5-triphosphate, 10 mM phosphocreatine, final pH 7.2, osmolarity of 270 to 290 mosmol

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constructed selfadjuvanting selfassembled nanoparticles by fusion of a trimerforming peptide to CTB, displaying the SARSCoV2 receptorbinding domain (RBD) antigen [97]

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