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Chattergoon et al., 2012
The culture medium consisted of Advanced DMEM/F12 (Gibco, 12634010), GlutaMAX (Gibco, 35050061), 10 mM Hepes (Gibco, 15630080), 27 1X (Gibco, 17504044), N2 1x (Gibco, 17502001), 1 mM N-Acetyl-L-cysteine (Sigma-Aldrich, A7250), recombinant mouse Noggin (100 ng/ml
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(1) control group
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