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The resulting single-stranded cDNAs were used as templates in PCR reactions using gene-specific primers to amplify TGR (forward 5-CTATTTCCGTAGACGTCTGT-3 and reverse 5-AATACAGTTTCCTTCCCGTT-3) or GAPDH (forward 5-GTTTTGGTCGTATCGGGAGA-3 and reverse 5-ATGCGTTAGAAACCACGGAC-3)

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The assay is based on reversed-phase high performance liquid chromatography with UV detection and simultaneous reduction of disulfide bound with tris(2-carboxyethyl)phosphine and the selective pre-column derivatization of the thiol group with 1-benzyl-2-chloropyridinium bromide