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The Food and Drug Administration (FDA) and Center for Biologics Evaluation and Research (CBER) define an IVD as: In vitro diagnostic products are those reagents, instruments, and systems intended for use in the diagnosis of disease or other conditions, including a determination of the state of health, in order to cure, mitigate, treat, or prevent disease or its sequelae

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In our application the number of correlations consisted of red fluorescent- tagged actin spines against green PSD-95 immunopositive puncta which, when merged, resulted in an orange spine