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Cells were added to high precision #1.5 coverslips (Corning, Millipore Sigma), placed on a 2% (w/v) agarose pad in LB and imaged at 37 C on a Nikon Ti inverted microscope equipped with a 561-laser line, a Plan Apo 100x/1.4 Oil Ph3 DM objective and a Andor Zyla 4.2 Plus sCMOS camera

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