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They were maintained under standard laboratory conditions like temperature 22-24 o C, relative humidity was 40-60%

Signs include: Swelling in the face or lips Trouble breathing Skin rash spreading quickly 4

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Notably, protein phosphatase 2A (PP2A) and AMP-activated protein kinase (AMPK) modulate the phosphorylation status of Beclin-1 to regulate autophagic flux (Peris et al., 2023)

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Ice formation and cracking was avoided through optimized convective cooling, and successful vitrification was confirmed via visual inspection, thermometry, and X-ray CT