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The hippocampus is rich in mGluR2 [91], and animal studies have shown that coronaviruses can induce neurodegeneration in the hippocampal CA1 and CA3 regions, leading to reduced short-term learning abilities and spatial memory impairment [92]

Bacterial expression of NS3/4A To express NS3/4A in Bacteria, BL21(DE3) cells were electroporated with pET16 NS3/4A JFH1 wt/D1467G and plated on Carbenicillin-containing Agar plates

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