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After 48 h, cells were lysed in cell-lysis buffer for western blotting and immunoprecipitation (Beyotime) with 1% protease-inhibitor cocktail (P8340, Sigma)

In PCa, the enlarged prostate obstructs the urethra, leading to difficulties in urination
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In the present study, apoptosis was closely related to glycolytic enzyme inhibition

Jorgensen A, Frikke-Schmidt R, Nordestgaard B, Tybjaerg-Hansen A

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However, half of the patients were level 3, indicating that they have begun taking action and viewed themselves as part of their own healthcare team