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For imaging, live worms were viewed on 2% agar pads, anesthetized with 10 l 2 mM levamisole

These controls allow researchers to accurately attribute observed effects to the combined stack and differentiate true synergy from additive effects or individual peptide contributions

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These findings support the important role of the microbiota-gut-brain axis in regulating systemic homeostasis, notably in the central nervous system

RDGF or retina-derived growth factor