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After adjusting the protein concentration using RIPA buffer, an equal amount of total protein (20 g) was resolved on 5% acrylamide gels (Amresco), and proteins were electrophoretically separated on SDS gels (1015%, Sigma) at a constant voltage of 120 V for 20 min ( n = 6 per group)

Unlike melatonin, which mainly regulates sleep-wake timing, DSIP seems to impact sleep depth and quality

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Funding This study is supported by the University of Zagreb, Zagreb, Croatia, grant number 099

Beharier O, Tyurin VA, Goff JP, Guerrero-Santoro J, Kajiwara K, Chu T, Tyurina YY, St Croix CM, Wallace CT, Parry S, Parks WT, Kagan VE, Sadovsky Y (2020) PLA2G6 guards placental trophoblasts against ferroptotic injury