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YCZ and PTZ made significant intellectual and technical contributions, including conceptualizing the study, conducting the initial literature review, refining the core content and designing the manuscript structure

Activation of transcription factors: P-DSIP can induce the expression of transcription factors such as c-Fos and CREB, transmit membrane receptor signals to the nucleus, and regulate gene expression

Muscle-relaxing peptides produce modest 20-40% wrinkle depth reduction based on limited clinical studies and extensive anecdotal reports, proving most effective for mild to moderate expression lines while showing minimal impact on deep established wrinkles requiring more intensive intervention